REVOLUTIONARY MALARIA MALARIA THERAPY USING ALLICIN IN GARLIC EXTRACT( ALLIUM SATIVUM): BIOMOLECULAR STUDIES USING IN SILICO
Donni Santoso 1, Gabriela Nativity 2, Putu Ijiya Danta Awatara 3
University of Brawijaya
Background: Malaria is life-threatening disease caused by Plasmodium Falciparum. In 201, half of world population about 3.2 billion people were at risk of malaria. Progress in malaria control is threatened by the rapid development and spread of antimalarial drug resistance. Allicin extracted from Garlic( Allium sativum) is biologically active component to inhibit cell proliferation and induce apoptosis.
Objective: To show microtubule of Plasmodium Falciparum plays important role in reducing malaria virulence and to show Allicin blocks these phenomenons.
Method: Biomolecular experimental is conducted by in silico. Comprehensive literature search conducted to prove the consistency of in Silico. Inclusion criterias were used to filter abstracts and full texts in assessing eligibility of trials.
Results: The result of in silico shows Allicin strongly binds and inactivates beta-tubulin microtubule protein of Plasmodium sp. in their active site, Alanine 206 through Alkyl bond. The binding affinity among allicin and microtubule protein is-3.7 kcal / mol as the highest and the lowest-3.3 Kcal / mol. All literature studies shown positive result towards Allicin inhibits Plasmodium Falciparum. Allicin can block cell division by preventing spindle formation resulting inhibiton of sporozoite invasion and infectivity of Plasmodium Falciparum.
Conclusion: Allicin obtained from Garlic can be revolutionary malaria therapy by inhibiting microtubule of Plasmodium Falciparum. Allicin strongly binds and inactivates beta-tubulin protein in their Active site and block Cell Division by Preventing Spindle Formation resulting inhibition of Sporozoite invasion and infectivity of Plasmodium Falciparum. Allicin recommended for East Asian continent.
Keywords: Antimalaria, Plasmodium Falciparum., Allicin, Garlic, Microtubule, Insilico