Detergents
100
Organic solvent that disrupts
Yielded cell removal and mild ECM
protein–protein interactions
disruptionwith Triton X-200
Sulfobetaine-10 and 16 (SB-10,SB-16)
Tri(n-butyl)phosphate
Variable cell removal; loss of collagen
Cell lysis by osmotic shock
content, although effect on mechanical
properties was
Minimal
Hypotonic and
hypertonic
solutions
Chelating agents that bind
Efficient for cell lysis, but does not
divalent metallic ions, thereby
effectively remove the cellular remnants
disrupting cell adhesion to ECM
EDTA, EGTA
No isolated exposure, typically used
with enzymatic methods (e.g., trypsin)
Enzymatic
Trypsin
Cleaves peptide bonds on the C-
Prolonged exposure can disrupt ECM
side of
structure,
Arg and Lys
removes laminin, fibronectin, elastin,
and GAGs
Endonucleases
Catalyze the hydrolysis of the
Difficult to remove from the tissue and
interior
could
bonds of ribonucleotide and
invoke an immune response
deoxyribonucleotide chains
Exonucleases
Catalyze the hydrolysis of the
terminal
bonds of ribonucleotide and
deoxyribonucleotide chains
18