Decellularization and Recellularization Technique
Tissue enginering holds a great potential to end the heart donor crisis. There is
one potential method which currently developed is decellurization and recellulerization of
whole-organ. Decellulerization is a protocol to efficiently remove all cellular and nuclear
material while minimizing any adverse effect on the composition, biological activity, and
mechanical integrity of the remaining ECM in the simple words decellulerization is any
processing step intended to remove cells will alter the native three-dimensional
architecture of the ECM. Component of ECM are generally conserved among species and
are tolerated well even by xenogonic recipient (Long et al., 2014).
Biologic scaffolds derived from decellularized tissues and organs have been
successfully used in both pre-clinical animal studies and in human clinical applications.
Removal of cells from a tissue or an organ leaves the complex mixture of structural and
functional proteins that constitute the extracellular matrix (ECM). The tissues from which
the ECM is harvested, the species of origin, the decellularization methods and the
methods of terminal sterilization for these biologic scaffolds vary widely. Each of these
variables affects the composition and ultrastructure of the ECM and accordingly, affects
the host tissue response to the ECM scaffold following impla