advantages and disadvantages of gel filtration chromatography 5

Gel Filtration Chromatography
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Gel filtration, as known as size exclusion chromatography (SEC), separates
proteins according to their different size as they pass through a gel filtration
column. Compared to affinity chromatography or ion exchange chromatography,
proteins to be seperated by gel filtration chromatography do not need to bind to
the chromatography medium, making buffer composition hardly affect resolution.
The major advantages and disadvantages of gel filtration chromatography are
listed as below:
There are three main applications of gel filtration chromatography:
1. In group separations
Adjusting pH, buffer type, salt concentration during sample preparation;
Removing interfering small molecules (EDTA, Gu-HCl, etc);
Removing small reagent molecules (fluorescent labels, radioactive markers, etc);
But not when the protein will precipitate.
2. In fractionation
Excellent during the polishing stage;
Removes dimers and other aggregates;
Transfers protein to buffer solution ready for the next set of experiments;
Not so suitable if the sample volume is large.