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Carmel Sparke IMMUNOLOGY and allergy specialists
are calling for shellfish skin prick tests to be standardised , with an analysis showing wide variability in the allergen extracts used in available tests .
Researchers — including paediatric immunologists from The Children ’ s Hospital at Westmead , Sydney — say skin prick testing ( SPT ) is usually the preferred first-line diagnostic approach . But widely used allergen extracts in commercial SPT kits are generally not standardised , limiting
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the diagnostic value of the results and potentially putting lives at risk .
Their study tested 11 crustacean and five mollusc commercial SPT extracts using biochemical and immunological methods and mass spectrometry .
In a research letter , published in Allergy , they said they found that some extracts lacked a sufficient amount of protein and contained a limited diversity of shellfish allergens , which could result in false negatives .
Total protein content in extracts
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varied by up to 14-fold ( 0.1-1.4mg / mL ) in five shrimp , two lobster , two oyster and three clam / scallop extracts from six different manufacturers .
In addition , using serum from five shellfish-allergic patients , immunoglobulin E ( IgE ) -binding patterns to 14 out of 16 extracts “ underlined high variance in anticipated in-vitro and in-vivo potency ”, according to the team led by James Cook University , Queensland .
In the case of one shrimp extract and one clam extract , no IgE binding
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was observed at all .
“[ This was ] likely because of low protein and allergen content suggesting a high risk of false-negative SPT results with these extracts ,” the authors wrote .
Lead author Dr Thimo Ruethers ( PhD ), from James Cook University ’ s Singapore campus , said improvements in blood tests , along with the development of region-specific allergen extracts , were “ critical ” to achieve better allergy testing . Allergy 2023 ; 21 Aug .
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