Acta Dermato-Venereologica, issue 9 97-9CompleteContent | Page 18

1125 SHORT COMMUNICATION No Viral Transcripts Associated with Folliculotropic Mycosis Fungoides Using a High Throughput Sequencing Approach Aurélie DU-THANH 1,2 , Olivier DEREURE 1,2 , Justine CHEVAL 3 , Marine DUMAREST 4 , Liza AL-SHIKHLEY 4,5 , Céline GIRARD 1,2 , Bernard GUILLOT 1,2 and Marc ELOIT 3,4 Department of Dermatology, Montpellier University Hospital, Hôpital Saint-Eloi, 80 avenue Augustin Fliche, FR-34295 Montpellier cedex 5, INSERM UMR 1058 “Pathogenesis and Control of Chronic Infections”, University of Montpellier, Montpellier, 3 Pathoquest, 4 Institut Pasteur, Biology of Infection Unit, Inserm U1117, Pathogen Discovery Laboratory, Paris, and 5 UMR BIPAR, INRA, ANSES, ENVA, Maisons-Alfort, France. E-mail: [email protected] 1 2 Accepted Apr 27, 2017; Epub ahead of print Apr 27, 2017 The involvement of infectious agents in the pathogenesis of mycosis fungoides (MF) is debated, although no pri- mate T-cell lymphotropic viruses nor previously known or unknown viral exogenous sequences were detected in 30 patients with classical MF (cMF) using semi-nested DNA amplification (1) or in 3 cMF patients with RNA- based high-throughput sequencing (HTS) (2), respecti- vely. Retroviruses and members of the herpesvirus family have largely been investigated in MF, with contradictory results (3). Cutaviruses were recently detected by PCR in 4/17 patients with cMF, but in situ hybridization revealed only rare positive cells, arguing against an oncogenic role (4). Folliculotropic MF (fMF) displays specific clinical characteristics, a deep lymphocytic infiltrate aggressing hair follicles, and a possibly less favourable outcome than cMF. Interestingly, the presence of Merkel cell polyomavirus (MCPyV) DNA was identified by real- time PCR in 50–75% vs. 13% of patients with fMF and cMF, respectively, in 2 series, using both formalin-fixed paraffin-embedded skin biopsies (5) and fresh-frozen tis- sues (6), with a higher viral load in fMF compared with cMF, T-cell mediated benign skin infiltrates (psoriasis, eczemas, etc.), or hea lthy individuals’ skin biopsies (6). Owing to these preliminary results and considering that follicles might represent potential reservoirs for various infectious agents, RNA-based HTS was performed on skin biopsies from 6 patients with fMF. METHODS Written informed consent was obtained from all patients and the experiments were conducted in accordance with the ICH GCP. Fresh-frozen 4-mm skin biopsies from characteristic skin lesions located on the trunk or lower limbs were obtained in 6 male patients (mean age 51.5 years, range 26–76 years) diagnosed with fMF stage IA (2 patients), IB (3 patients) and IIB (1 patient). Total RNAs were extracted with Trizol from skin biopsies, reverse transcribed and randomly amplified to high molecular weight DNA, as described previously (7). Library preparations and sequencing with an Illumina HiSeq2000 sequencer were outsourced to DNAVision (Charleroi, Belgium). HTS analysis for the presence of viral RNA (e.g. transcripts of DNA viruses and/or genome/transcripts of RNA viruses) was conducted as reported previously (8). Endogenous retroviral sequences different from those harboured by the prototypal hG19 human sequence are detected by this method. However, they are not reported, because they are part of the human DNA: thus, they are different from exogenous viruses targeted by this method. RESULTS AND DISCUSSION No known or unknown viral sequences could be detec- ted by the powerful methods used in this study. These negative results first raise the issue of the sensitivity of RNA-based HTS, reminiscent of the very low viral load of MCPyV, which was established at less than 0.5 copies/ cell, and 0.002–12.467 copies/beta-globin gene copy in previous studies (5, 6). However, it must be pointed out that the same method has already succeeded in detecting viral sequences shown by RT-qPCR to be in very low amount, which is an indirect evidence of its high level of sensitivity (7). Furthermore, the relevant negative predictive value of our results regarding the presence of viral transcripts is attested by the coverage of rare cellular transcripts (Table SI 1 ). Although based on only 6 skin samples, these prelimi- nary data do not support the hypothesis that RNA viruses are significantly involved in fMF. Similarly, the lack of detection of transcripts from DNA viruses strongly suggests that they are genuinely absent or that the level of transcription is below the sensitivity threshold of the method. However, DNA viruses might be involved in fMF through other pathways than viral antigen expres- sion, such as insertion-driven mutations of the host cell genome by few bases or full viral genome, as non- transcribed sequences that would not have been detected in this study. Nevertheless, such insertion mutagenesis has not been described for DNA viruses in cancer (9). From this perspective, it is of importance to point out that, unlike previous reports based on DNA, the present study was performed on total RNAs extracted from total skin biopsy samples, a method precluding the detection of non-replicating skin-associated DNA viruses that could contaminate skin biopsies. Thus, DNA-based HTS, along with microdissection techniques targeting tumoural cells, might significantly improve the sensitivity of this investigation and the robustness of its conclusions. ACKNOWLEDGEMENTS This work was partially funded by Laboratoire d’Excellence ‘Integrative Biology of Emerging Infectious Diseases’ (grant no. ANR-10-LABX-62-IBEID). https://www.medicaljournals.se/acta/content/abstract/10.2340/00015555-2686 1 This is an open access article under the CC BY-NC license. www.medicaljournals.se/acta Journal Compilation © 2017 Acta Dermato-Venereologica. doi: 10.2340/00015555-2686 Acta Derm Venereol 2017; 97: 1125–1126