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Featured in this month’ s issue:
CKIP-1 silencing promotes new bone formation in rat mandibular distraction osteogenesis Zi-chao Zhou, Lei Che, Liang Kong, De-lin Lei, Rui Liu, Xin-jie Yang
Objective
This study investigated the effects and possible molecular mechanism of casein kinase-2 interacting protein-1( CKIP-1) silencing on bone regeneration during rat mandibular distraction osteogenesis( DO).
Study Design
CKIP-1 silencing by chitosan / si-CKIP-1 was employed and analyzed both in rat mandibular DO models in vivo and in cultured rat mandible bone marrow stromal cells( BMSCs) in vitro.
Results
Gross observation, micro – computed tomography analysis, and hematoxylin and eosin( H & E) staining revealed that new bone formation in the distraction gap of the chitosan / si-CKIP – treated group was better compared with the chitosan / si-NC and phosphate buffered saline – treated groups in both quantity and quality. Proliferation assay, flow cytometry, and alizarin red staining indicated that CKIP-1 silencing significantly inhibited apoptosis, but promoted osteogenic differentiation of cultured BMSCs. Additionally, CKIP-1 silencing significantly promoted the expression of Wnt3 a, β-catenin, and osteocalcin both in new bone formation of DO models in vivo and in the osteogenic differentiation process of BMSCs in vitro.
Conclusions
Promotion of bone formation after CKIP-1 silencing in rat mandibular distraction osteogenesis appears to be mediated through the Wnt3 a / β-catenin signaling pathway.
Also in this month’ s issue:
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Differential modulation of zoledronate and etidronate in osseous healing of an extracted socket and tibia defect Shin Saeng Lim, Beomseok Lee, In Sook Kim, Soon Jung Hwang
Osteoblast-like cells with different embryologic origins behave differently in increasing zoledronic acid concentrations: a pilot study in pigs Philipp Kauffmann, Wolfram Hahn, Niklas Sievers, Markus Troeltzsch, Karin Hohloch, Phillipp Brockmeyer, Robert Cordesmeyer, Henning Schliephake, Rudolf Matthias Gruber
Stability of large maxillary advancements using a combination of prebent and conventional plates for fixation Marwa Ragaey, Joseph E. Van Sickels
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